5. Conclusions
An understanding of the irreversible immobilization is important to tune appropriate immobilization conditions for the fabrication of biomolecular surfaces. In this paper, the covalent immobilization of bovine serum albumin (BSA), lipase from C. rugosa and protein G on differently functionalized regenerated cellulose membranes was successfully achieved by using different chemical surface modifications.
Immobilization kinetics trends indicated that the saturation coverage of BSA and protein G was concentration independent, and moreover their binding isotherms, parallel to the concentration axis over the concentration range studied, suggested a non-equilibrium irreversible process. AFM micrographs of BSA and protein G functionalized membranes give indication about the formation of monolayer, which reflect the oligomers formed in solution.