2. Materials and methods
2.1. Plant materials
Nodal cuttings (with one leaf) of potato plantlets were cultured
in 0.6% agar solidified MS (Murashige and Skoog, 1962) medium
containing 30 g/L sucrose (pH 5.8); incubated at 25
◦
C and 50–55%
relative humidity, and subjected to 50mol m−2
s
−1
light intensity
with 16 h photoperiod. After 3 weeks of culture, each shoot was
divided into nodal segments, each containing one node, and subcultured in a similar medium