Alkyd resins have been commonly used as binders in artist paints since the 1940s. The characterizationof alkyds in samples from artworks can help to solve attribution and dating issues, investigate decay pro-cesses, and contribute to the planning of conservation strategies. Being able to assess the components ofindustrially formulated paint materials and to differentiate between different trademarks and producersis extremely interesting and requires multi-analytical approaches.In this paper we describe the characterization of commercial alkyd paint materials using a multi-analytical approach based on the integration of three different mass spectrometric techniques: gaschromatography–mass spectrometry (GC/MS), high performance liquid chromatography coupled withelectrospray ionization mass spectrometry with a tandem quadrupole-time of flight mass spectrometer(HPLC–ESI-Q-ToF), and flow injection analysis (FIA) in the ESI-Q-ToF mass spectrometer.GC/MS was successful in determining the fatty acid and aromatic fractions of the resins after hydrolysis;HPLC–ESI-Q-ToF analysis enabled us to identify the triglycerides (TAGs) and diglycerides (DAGs) profileof each resin, and FIA analysis was used as a rapid method to evaluate the presence of possible additivessuch as synthetic polymers.