2.1.4. Enzymes
Three different enzymes were selected on the basis of the structural
composition of grape skins and seeds. Cellulase (P700U/g,
E.C. 3.2.1.4) from Trichoderma reesei was purchased from Sigma–
Aldrich (St. Louis, MO). Pectinase (3500 U/g, E.C. 3.2.1.15) from
Asperigillus niger and ß-glucosidase (1000 U/g, E.C. 3.2.1.21) from
Sweet almonds were purchased from MP Biomedicals LLC (Solon,
OH). All enzyme reactions were performed in a 0.2 M sodium acetate
buffer (pH 4.8).