3. Results and discussion
In highly processed food products such as gelatin and gelatin containing food and pharmaceutical products, DNA is degraded into short fragments. This issue caused some difficulties in PCR amplification as reported by earlier researchers (Mafra, Ferreira, & Oliveira, 2008; Martín et al., 2007). The essential prerequisite for PCR amplification is therefore obtaining sufficient template DNA for analysis. For this reason, DNA isolation was performed using a commercial kit under optimized columnbinding conditions adjusted for maximal recovery of short DNA fragments.
3. Results and discussionIn highly processed food products such as gelatin and gelatin containing food and pharmaceutical products, DNA is degraded into short fragments. This issue caused some difficulties in PCR amplification as reported by earlier researchers (Mafra, Ferreira, & Oliveira, 2008; Martín et al., 2007). The essential prerequisite for PCR amplification is therefore obtaining sufficient template DNA for analysis. For this reason, DNA isolation was performed using a commercial kit under optimized columnbinding conditions adjusted for maximal recovery of short DNA fragments.
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