Protein structure was characterized using differential scanning calorimetry
(DSC), Fourier-transform infrared (FTIR) spectroscopy, and circular dichroism (CD) spectroscopy. The
ELISA kits performed well in detecting allergens in the unprocessed flours with recoveries ranging from
63–120%. The results for dough and uncooked pasta were similar to those of unprocessed flours. Recoveries for
boiled pasta were significantly (p ≤ 0.05) reduced.