Commercial grade sample of P. nigrum was purchased from super market, Yerkaud, Salem, India. P. nigrum fruit berries were dried and ground to yield powder of 100 mesh size parti- cles and were directly subjected to extraction. Methanol, hex- ane, petroleum ether, ethyl acetate, diethyl ether and silica gel (60–120 mesh for CC and 200–400 mesh for TLC) were purchased from S.D. Fine Chemicals, Mumbai, India.DBSA. All extractions were continued until colorless. Percent- age extraction yield (w/w) for crude was obtained by using the formula