2.2.3. Triacylglycerol composition
The triacylglycerol composition was analyzed according to the
AOCS Ce 5-86 method (AOCS, 2009) using a gas chromatograph
(GC CGC AGILENT 6850 Series GC) system and a capillary column
(DB-17 HT AGILENT; 50% phenylmethylpolysiloxane, 15 m long,
0.25 mm internal diameter, 0.15 mm film thickness). The analysis
conditions were as follows: split ratio 1:100; column temperature:
250 C programmed up to 350 C at a rate of 5 C/min, carrier gas:
helium at a flow rate of 1 mL/min; injector temperature: 360 C;
detector temperature: 375 C; injection volume: 1 lL; sample concentration:
100 mg/5 mL of tetrahydrofuran. The triacylglycerol
groups were identified by comparing the measured retention times
with those reported by Antoniosi Filho, Mendes, and Lanças (1995).
The analyses were performed in triplicate.