The concentrations of SM used in the preliminary experiment
were 250 g/L, 333 g/L and 500 g/L. The experiments were conducted
in the bioreactor for 12 h at 35 ◦C and 230 rpm and used 35 g/L
inoculum of S. cerevisiae and 0.050% enzyme. The results are shown
in Table 7.
Upon analysis of the results in Table 7, we observed that the
highest yields (Y2) were obtained with lower concentrations of soybean
molasses. Thus, the concentration of 250 g/L was adopted for
the next set of experiments.