The total count of hemocytes (TCH) was significantly higher
(p b 0.05) in the group fed with a diet supplemented with the probiotic
Bacillus spp. (1.15 × 104 CFU·g−1) compared to the control (Fig. 2a),
confirming the results reported by Li et al. (2007a), who added
B. licheniformis in three concentrations to the L. vannamei culture
water. According to Jiravanichpaisal et al. (2006), increased THC can
provide greater protection of crustaceans against infection, since hemocytes
are responsible for the different immune cell reactions.
The concentration of total serum protein (CP) was significantly
higher (p b 0.05) in the group treated with the diet supplemented
with the probiotic Bacillus spp. compared to the control (Fig. 2b).
Gullian et al. (2004) and Li et al. (2007a) found no significant difference
in total protein concentration in the plasma of L. vannamei shrimp fed
with Bacillus sp.
The phenoloxidase (PO) activity showed statistical significance
(p b 0.05), being lower in the group treatedwith the diet supplemented
with probiotics (Bacillus spp.) compared to the control (Fig. 2c). Contrary
to the results found, Gullian et al. (2004) and Li et al. (2007a) observed
an increase in phenoloxidase activity in shrimp fed with
probiotics, just as Chiu et al. (2007), Li et al. (2009), and Tseng et al.
(2009) reported an increase in the phenoloxidase activity of groups
treated with probiotics only after challenging the shrimp with Vibrio
spp. andWhite Spot Syndrome Virus (WSSV). Therefore, it is important
The total count of hemocytes (TCH) was significantly higher(p b 0.05) in the group fed with a diet supplemented with the probioticBacillus spp. (1.15 × 104 CFU·g−1) compared to the control (Fig. 2a),confirming the results reported by Li et al. (2007a), who addedB. licheniformis in three concentrations to the L. vannamei culturewater. According to Jiravanichpaisal et al. (2006), increased THC canprovide greater protection of crustaceans against infection, since hemocytesare responsible for the different immune cell reactions.The concentration of total serum protein (CP) was significantlyhigher (p b 0.05) in the group treated with the diet supplementedwith the probiotic Bacillus spp. compared to the control (Fig. 2b).Gullian et al. (2004) and Li et al. (2007a) found no significant differencein total protein concentration in the plasma of L. vannamei shrimp fedwith Bacillus sp.The phenoloxidase (PO) activity showed statistical significance(p b 0.05), being lower in the group treatedwith the diet supplementedwith probiotics (Bacillus spp.) compared to the control (Fig. 2c). Contraryto the results found, Gullian et al. (2004) and Li et al. (2007a) observedan increase in phenoloxidase activity in shrimp fed withprobiotics, just as Chiu et al. (2007), Li et al. (2009), and Tseng et al.(2009) reported an increase in the phenoloxidase activity of groupstreated with probiotics only after challenging the shrimp with Vibriospp. andWhite Spot Syndrome Virus (WSSV). Therefore, it is important
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