The fourth experiment (E4) consisted in one patch of R. oryzae inoculum (Ø: 1 cm, h: 4 mm) placed to one side of a sterile polystyrene Petri dish, and the rest of the Petri dish
filled with 150 glass beads (Ø: 5 mm) embedded with a layer of enriched agar, and once solidified, a second layer of microalgae lipids was added (1177 cm2 of total area and 6397 mg of enriched agar media).