affected the formation of multiple shoots on cultured shoot tips from
a rubber tree, reducing the average number of shoots per explant when
compared with the control medium, although the effect was only significant in
respect of the peptone supplemented medium. The formation of multiple shoots
was detected after 3 weeks of being cultured in SIM containing peptone.
Between 93 and 100% of the explants cultured in SIM supplemented with all
concentrations of peptone produced shoots without significant difference
between explants raised in media containing different concentrations of
peptone, in either the percentage in which multiple shoot formation occurred or
the number of shoots produced per explant (Table1) There was however a
significant difference between the number of shoots produced between the
control medium and the peptone-supplemented media. In addition, some shoots
were small and wilted after 4 weeks of being cultured (Fig. 1b)