C- Screening for antimicrobial activity of probiotic metabolites:
The antibacterial activity of lactic acid bacteria was determined by following the well agar diffusion
method. (Schillinger and Lncke 1989) agar was transferred to the Petri dish and allowed to solidify. An aliquot
of 0.1 ml of pathogenic bacteria fresh culture suspension was transferred to plate count agar and spread
uniformly over the agar surface with a sterile bent glass rod. Plates were dried at 370 C for 1hr.
The supernatant of probiotic culture 20μl were added into filter paper discs after neutralization to pH
6.0.The inhibition zone formed around each disc , which indicate the antibacterial activity was measured in mm.