Cell proliferation on Ormocers surfaces processed
using 2PP was examined using human epidermal keratinocytes.
The 3-(4,5-dimethylthiazol-2-yl)MTT test
was performed using a modification of the method described
by Mossman.34 This assay is based on reduction
of a yellow tetrazolium salt (MTT) to a purple formazan
dye by mitochondrial succinnic dehydrogenase. The
Ormocers was placed between two 18mm18mm
coverslips. This construct was carefully wedged apart
with a razor blade, and the polymer adhered to one of
the coverslips. The remaining coverslips were used as
control surfaces. Three wells in the plate contained