he efficacy of a test dentifrice containing
nano-sized (several tens to hundreds of nm) calcium
carbonate (hereafter NC) on enamel lesion
remineralization was studied in an
in vitro
system that
employed collagen-coated wells for cell culture, as a
model of oral surfaces for NC retention. The well
surfaces were treated with the test dentifrice and briefly
rinsed with distilled water.