For the preparation of the extracts, leaves were collected which
were kept submersed in methanol and hexan separately for 72 h;
afterward, the extract was filtered and concentrated using a rotary
vacuum evaporator (model Q-344B–Quimis, Brazil) and ultrathermal
bath (model Q-214M2–Quimis, Brazil), obtaining yields of
crude extracts presented in Table 2. The solution utilized in the tests
was prepared at a concentration of 10 mg/ml, dissolved in DMSO
and then diluted with distilled water to obtain a concentration of
1024g/ml.