In this study, the correlation between tumor suppressor gene compositional features and its expression level has been revealed. Since dysregulation of tumor suppressor genes in normal tissues might lead to tumor formation and progression, any knowledge about themechanism of the regulation of these genes can help us to understand cancer better. The significant correlations between sequence features and mRNA expression level of tumor suppressor genes have important implications for understanding the molecular mechanisms of tissue-specific oncogenesis. Also, these results could confirm some evidence obtained from other studies.