According to the validated parameters of accuracy, precision, linearity, LOQ and LOD, the proposed technique was successful in simultaneously and quantitatively analyzing four major mangostin derivatives (3-isomangostin, gamma-mangostin, beta-mangostin, and alpha-mangostin) in propolis extracts of T. pagdeni from mangosteen plantation. This method would be beneficial in the quality control and the standardization of propolis extracts and preparations from mangosteen orchard.