2.5. Bioactive compounds analyses
2.5.1. Vitamin C
Vitamin C was extracted, separated, identified and quantified
by HPLC according to Rodríguez-Roque et al. (2013a). Vitamin
C identification was carried out by comparing its retention time
and spectra with the standard (ascorbic acid) and using a calibration
curve (R2 = 0.9989, at the concentration range of 10
to 1000 mg/L). Results were expressed as mg of ascorbic
acid/100 mL of sample.