After proper in vitro stabilization on plant growth regulator-free (PGR-free) Murashige and Skoog (MS) basal medium, earliest shoot bud regeneration was obtained on a medium containing full strength MS inorganic salts, 100 mg/l myo-inositol, 0.1 mg/l thiamine-HCl, 3% sucrose, 0.8% agar as gelling agent and supplemented with 0.25 mg/l 6-Benzylaminopurine (BAP).