Another possible reason
could be nutrient rich diet being fed to females
during the pregnancy period and the same (nutrient
rich) food is being utilized by the parasites thereby
resulting in more growth. It was also observed that
fixation had a direct effect on the size of the body
and other body organs as the parasites fixed in too
hot or too cold fixative shrink to different lengths as
compared to those fixed in alcohol with optimum
temperature. The reason could be that too hot
fixative cause’s shrinkage of the parasite while as
the parasite does not stretch fully in cool fixative
and in both the cases the parasite appears shorter.
However the coloration was slightly changed
depending on the food of the host. The
comparatively small variation in the size of the
present specimens could be due to the effect of
environmental factors of the study area as it is the
only cold desert of the World, where the
environment is all together different or due to some
other unknown reasons