Microbial analyses of control and treated juice samples were performed by using the standard method mentioned in the FDA's Bacteriological Analytical Manual(FDA, 2001) Total plate count was determined by pour plate method. Sterilized distilled water was used to make serial dilutions which were then poured into sterile petri plates after decimal dilution(up to 10 5) of sample Molten agar(15 mL)was added to each petri dish and the mixing of samples were done five times in directions vertically, clockwise and horizontally, anti-clockwise. The petri dishes were then allowed to set at 25 1 C for 30 min. The dishes were then shifted to an incubator(GSP-9080 MBE, Shanghai Boxun Industry& Commerce Co. Ltd, China) for 2 days at 32 by turning the dishes upside down. Colonies in juice samples were counted(as CFUlmLjuice) by multiplying with reciprocal. The results were expressed as log colony forming units(CFUlm of sample Pour plate method was used to determine the total yeast and mold counts. Known amount(39 g) of potato dextrose agar(PDA) powder was dissolved in 1000 mL of distilled water to prepare media. To avoid the cross contamination, tartaric acid solution(10:90, wlv) was added in the PDA. All the petri dishes containing PDA were placed in an incubator at 32 t 1 °C. Then after two days