Inhibitory activities of test antagonists on the
mycelium growth of P. ostreatus in a submerged
culture
Ten millimeters of PDB was dispensed into a screw
capped test tubes and were sterilized at 121 °C for 15 min
and allowed to cool before inoculation. Five millimeters of
stock medium (mycelium in broth culture) and the test
organisms (antagonists) each were inoculated into the test
tubes. The treatment was carried out in triplicate while the
control tube was left without any inoculum. Growth in
terms of turbidity was measured on daily basis for 168 h at
540 nm wavelength using spectrophotometer
(Spectrumlab 22pc model).