3.5. Detection limit
The detection and quantification limits as defined by IUPAC
[34],CLOD =3Sb/mandCLOQ =10Sb/m(where Sb is the standard
deviation of the blank andmis the slope of the calibration graph)
were found to be 2.0 and 6.6 ng ml−1, respectively. The slope of
the calibration graph (m) is the calibration sensitivity according
to IUPAC definition.