Delgado et al. (2013) concluded that not only volatiles, but also macronutrients and/or biological molecules with important physiological roles were affected by the intensity of pressure and temperature utilized in a HPT processing. According to the aforementioned study, only the treatments at 300 MPa and 50 °C could be suitable for the preservation of essential nutrients and immunological components of breast milk. However, although initial temperature of processing was moderate (50 °C) and despite the fact that the holding time is shorter in HPT processing compared to HoP (30 min), the increase of temperature due to the adiabatic heating reached ~ 67 °C, temperature being even higher than 62.5 °C applied in HoP. The increase of temperature during processing could be responsible of the degradation effect of treatments.