In the present study a thematic attempt was made to investigate the effect of various parameters including moisture levels, pH, incubation temperatures, carbon sources as an additional growth supplements (glucose, fructose, sucrose, maltose, molasses), nitrogen sources as an inexpensive growth supplements (urea, yeast extract, beef extract, bacteriological peptone, ammonium sulfate), and inoculum size on pectin lyase production in SSF of lemon peel waste (selected from initial screening trial experiment) as most suitable substrate for pectinolytic enzymes production from A. oryzae.