The transesterification reaction catalysed by immobilized lipase was studied in this work, in order to verify the possibility of using it for the production of biodiesel from suitable wastes, such as vegetable oils. In particular, laboratory tests were carried out with the aim of evaluating the influence of some factors on reaction yields. Our biocatalyst – Epobond P. cepacia – was found to be very interesting in transesterification of fresh and waste vegetable oils. Moreover, the possible problems that occur in the transition from refined vegetable oils to waste oils were studied and it was observed that the the yields in esters did not vary significantly. Finally, it was verified that the biocatalyst can be used for multiple reaction cycles, even if it requires more investigations in order to evaluate and distinguish the effects of deactivation caused by washing and use in the reaction system.