It might be possible that, at the beginning of the storage, the probiotic could effectively use nutrients such as sugars, amino acids and organic acids in banana purée
for enhancing its growth and multiplication, thus cell colonization
occurred by adhering to surface of the purée particulates. This
could be proved in the past experiment (data not shown, but to be
reported elsewhere) which showed this probiotic bacterium,
grown in the specific medium added with this ‘Kluai Khai’ purée,
had the highest prebiotic activity score and, when kept in the
purée, there were increased numbers of viable cells resulting in
increased titratable acidity during storage at 4
C for 21 days. This
demonstrates that the probiotic bacterium used appropriate
nutrients including inulin (as a prebiotic) in the banana purée to
maintain its growth activities. In addition, lactic acid bacteria are
capable of producing exopolysaccharides (EPS) during their growth
and these materials are essential for surface adhesion