Cultivation of bacterial composition of Enterobacter aerogenes E13, Arthrobacter sp. N3 and Bacillus coagulans Š1 was carried out in flasks. The 750 mL Erlenmeyer flasks containing 70 mL of complex nutrient medium were incubated at 30 °C in a rotary shaker Innova 43 (New Brunswick Scientific Co.) at 200 rpm for 16 h. All 3 cultures of microorganisms were used in the same amount.
As model fatty substrate were set the most common by composition of fatty acids fats in wastes such as porky fat, beefy suet and sunflower oil in the same amount by weight. All experiments were divided into three major groups, i.e. the selection of optimal parameters of wastewater and pipelines biological treatments, and the determination of optimal parameters of composting process.