Plant extracts were tested for the scavenging effect on DPPH radical according to the method of Pan et al.19 0.2 mL of extract solution in ethanol (95 %) at different concentrations (0.2, 0.5, 0.8 and 1.2 mg mL-1) was added to 8 mL of 0.004 % (w/v) stock solution of DPPH in ethanol (95 %). The scavenging activity on the DPPH radical was determined by measuring
the absorbance at 517 nm until the reaction reached the steady state, using a UV–Visible TV- -1901 spectrophotometer (Beijing Purkinje General Instrument Co. Ltd., China). As a positive control, synthetic antioxidant BHT was used. All determinations were performed in triplicate. The DPPH radical scavenging activity (S%) was calculated using the following equation: S% = ((Acontrol – Asample)/Acontrol)×100, where Acontrol is the absorbance of the blank control (containing all reagents except the extract solution) and Asample is the absorbance of the test sample
Plant extracts were tested for the scavenging effect on DPPH radical according to the method of Pan et al.19 0.2 mL of extract solution in ethanol (95 %) at different concentrations (0.2, 0.5, 0.8 and 1.2 mg mL-1) was added to 8 mL of 0.004 % (w/v) stock solution of DPPH in ethanol (95 %). The scavenging activity on the DPPH radical was determined by measuring
the absorbance at 517 nm until the reaction reached the steady state, using a UV–Visible TV- -1901 spectrophotometer (Beijing Purkinje General Instrument Co. Ltd., China). As a positive control, synthetic antioxidant BHT was used. All determinations were performed in triplicate. The DPPH radical scavenging activity (S%) was calculated using the following equation: S% = ((Acontrol – Asample)/Acontrol)×100, where Acontrol is the absorbance of the blank control (containing all reagents except the extract solution) and Asample is the absorbance of the test sample
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