bacterial
bacterial chromosome
plasmid
cell containing gene of interest
gene of insert
DNA of chromosome
1. gene inserted into plasmid
Recombinant DNA (plasmid)
2. plasmid put in to bacterial cell
Recombinant bacterium
3. Host cell grown in culture to form a clone of cell containing the cloned gene of interest
protein expressed by gene of interest
gene of interest
copies of gene
protein harvested
4. Basic research and various applications
Basic research on gene
Basic research on protein
Gene for pest resistance inserted into plants
Gene useed to alter bacteria for cleaning up toxic waste
Protein dissovlves blood clots in heart attack therapy
Hunman growth hormone treats stunted growth