given there is no evidence that the digestive gland is an energy storage organ in
Sepioteuthis australis (Semmens, 1998).
The percentage water, protein, carbohydrate and lipid were determined using
techniques described in Moltschaniwskyj and Martinez (1998). Duplicates were run for
all tissue analyses for each individual, and blanks were also run simultaneously.
Concentrations of lipid, protein and carbohydrate were expressed as a function of the
wet weight of the muscle mass. Mantle muscle tissue was analysed for RNA
concentration using a Promega Total RNA isolation system kit (Cat. No. Z5110). RNA
content was expressed as a ratio of the protein concentration in the mantle muscle tissue.