3) Pressurissed Liquid Extraction: Extraction was done by using an ASE 200 accelerated solvent extractor (Dionex Ltd. Camberley, Surrey, UK). About 3 g of sliced lemongrass stem and 1 g of diatomaceous earth was accurately weighed and mixed together before being placed into the 22 mL cells with cellulose filter at the bottom end. The sample cell was closed to finger tightness before being placed into the carousel of the ASE 200 system. Sample was
extracted using n-hexane, using standard method as proposed by [14], which were a temperature of 100°C, pressure of 1000 psi and a 30 minutes time