10% (v/v) of D2O was added to the protein sample prepared solubilized
in 10 mM potassium phosphate buffer (pH 7.6) with
100 mM KCl and 0.05%DDM before spectral acquisition. NMR
experiments were performed on a Bruker AVANCE II 500 MHz
spectrometer equipped with a TXI probe. The 1H NMR spectrum
was acquired at 25 C with spectral width of 80 kHz, and processed
in the Topspin 3.2 software from Bruker using an exponential
apodization function.