A 10 mL of 0.005 M NaOH solution containing 15 mg of 2-HIYPMI was added to water solution containing 0.8 g alumina of 2-HIYPMI, and 30 mg of SDS in a 25 mL vial and pH was adjusted to 2.0. After shaking for 1 h, a portion of the supernatant liquid was diluted to the appropriate volume and the absorbance of the solution was measured at maximum wavelength (280 and 317 nm for 2-HIYPMI). Comparisons of the absorbance of supernatant solution with absorbance of initial ligand solution at similar conditions indicate the amount of ligand loaded on SDS-A. Ligand molecule will be entrapped in the interior core of the ad-micelles formed on the alumina surface lead to change in alumina colour that is an indication of ligand immobilization on SDS-A.