2.7. Thiol quantification
Protein thiol groups were determined by derivatization with DTNB
(Ellman, 1959). The analysis was conducted as described by Jongberg,
et al. (2013) with the only modification that the reaction volume was
downsized to 1 ml cuvettes instead of 3 ml cuvettes. The thiol concentrations
given as nmol thiol/mg protein are presented as means ± sd
of three independent replicates (n= 3).