The effects of lactic acid and EtOH on CBC-1 growth. Strain CBC-1 was grown to saturation overnight with aeration in YPD medium and back diluted into YPD medium lacking lactic acid (pH 7) or lacking lactic acid but supplemented with 8% EtOH (pH 7 + EtOH), as well as YPD medium acidified with lactic acid to pH 3 and 2.5 in the absence and presence of 8% EtOH. The cells were then incubated without aeration, and their growth was monitored by pipetting the cells into suspension and measuring the OD660. At pH 7 and 3, EtOH increased the lag phase of growth. It also decreased the doubling time of the cells grown in YPD-pH3 medium. At pH 2.5, no growth was evident. All experiments were performed ≥3 times on biological replicates, and data plotted as growth curves are the averages with the standard deviation as the error bars. แปล