Visualizing and analysis of PCR products
The amplification products were mixed with 1x
loading buffer (10mM Tris-HCl (pH:7.6), 10 mM
EDTA, Glycerol 10%, 0.005% bromophenol blue) and
separated using 1.5% agarose gel in 0.5X TBE buffer
and stained with ethidium bromide visualized using
a Fluor-S Multi Imager. The mw of each band was
estimated by comparing with a co-migrating known
size DNA ladder 100 bp (SMO321, Fermantas).