where: Wo,Wwwere the mass of pAAm-Sc initial and after 24 hof washing respectively. The data reported is the average of three measurements.
The amount of cells immobilized was calculated by subtractin the number of cells in the washing solution after 24 h, from the original number of cells added. Cells immobilization efficiency wasde fined as the weight percentage of yeast in a hydrogel that has been immobilized