This study evaluated the efficacy of lactic acid bacteria (LAB) isolated from traditional fermented foods and beverage of Ladakh as biocontrol agents against Yersinia enterocolitica. The antagonistic activities of 46 LAB isolates were screened for their ability to inhibit the growth of Y. enterocolitica at refrigeration temperatures using agar spot tests. On the basis of the 16S rDNA sequences, two selected inhibitory isolates were identified as Lactobacillus sp. and Lactobacillus brevis and their accession number were KJ722775 and KJ722777, respectively. The growth of Y. enterocolitica in mixed cultures coinoculated with Lactobacillus sp. and L. brevis separately in de Man Rogosa