2.3. Statistical analysis
Observations were made and all the experiments run in triplicates.
At least three separate flasks were maintained for one treatment. Each
time three readings were taken, and mean value and standard error
were calculated. Excel software was used to compile all results to form
a database. Basic descriptive statistics was calculated for all the growth
parameters of all samples. ANOVAwas obtained by using Duncan's multiple
test in order to have treatments comparison at 0.05 significance
level. All of the statistical procedureswere performed by using Statistics
software (version 8.1).