Scope—Three fluorescence biosensors were developed based on a 3T3-L1 preadipocyte line that
stably expressed Nfkb-RE/GFP, Fabp4-P/CFP, and Nrf2-P/YFP fluorescent reporters. We
hypothesized that nutraceuticals’ inflammatory, adipogenic, and antioxidant status will be
identified based on the change in fluorescence in reporter adipocytes. We validated these assays
with activators of NFκB, FABP4-regulating PPARγ, NFR2 and, thereafter, tested known and
unknown properties of mangosteens (MG), the xanthone metabolites in mangosteen fruit.