To simulate survival in the GIT, the 50 pre-selected LAB isolates were tested in an in vitro model that chemically replicates physiological conditions. In the tolerance to low pH test, the pH of MRS broth was adjusted to 2.0 with 1 N hydrochloric acid.
To simulate survival in the GIT, the 50 pre-selected LAB isolateswere tested in an in vitro model that chemically replicates physiologicalconditions. In the tolerance to low pH test, the pH of MRSbroth was adjusted to 2.0 with 1 N hydrochloric acid.