Water-in-mineral oil adjuvants induce a strong long-term humoral immune response and are widely used in poultry
vaccines. New adjuvants that also increase the cellular immune response could help to extend the vaccinal crossprotection
against different viral strains or serotypes. We have developed a new water-in-oil adjuvant, MontanideTM
ISA 71 VG, based on a specific enriched light mineral oil which stimulates both humoral and cellular immune
responses. Here, using a Newcastle Disease vaccine model, we demonstrate that this new adjuvant is safe, can
improve vaccine efficacy in poultry and could allow the reduction of injection doses of inactivated poultry vaccines.