Intra-assay
variabilities (¼coefficient of variation) for each dilution step for
nptII, nptIII and 16S were 0.19e1.97%, 0.1e5.15%, and 0.16e0.7%.
Inter-assay variabilities were 1.34e3.46%, 0.6e2.13%, and
0.58e2.14%. Specificity of primers and probes was verified by BLAST
analysis excluding all artificial (i.e. vector) sequences.