2.4. Studies in experimental screenhouse
Another experiment was performed to study the effect of Artemisia oil on 6-week-old weed plants raised under controlled conditions in experimental screenhouse.
Plants of all the test weeds were raised from the collected seeds in 12cm diameter earthenware pots. Pots were filled with 750 g garden soil (soil:sand:manure: 3:1:1, w/w) and seven seeds of each weed species were sown per pot.
Two-week after emergence, pots were thinned to 5 equal-sized healthy plants per pot. Plants were watered daily and after 3 weeks they were flushed with halfstrength Hoagland nutrient solution (Hoagland and Arnon, 1950).
When plants were 6-week-old, these were sprayed with 2, 4, and 6% (v/v) solution of Artemisia oil or distilled water (as parallel control) using a common garden sprayer at the rate of 100 ml/m2.
For each seed type, a total of 20 pots comprising of 4 treatments and five replicates per treatment were maintained in a completely randomized design.
One- and 7-days after spray (DAS), the treated
test weed plants were examined for visible injury levels in termsof percent chlorotic and necrotic areas.
Further, the leaves wereplucked for the determination of chlorophyll content and cellular respiratory activity.
2.4. Studies in experimental screenhouseAnother experiment was performed to study the effect of Artemisia oil on 6-week-old weed plants raised under controlled conditions in experimental screenhouse. Plants of all the test weeds were raised from the collected seeds in 12cm diameter earthenware pots. Pots were filled with 750 g garden soil (soil:sand:manure: 3:1:1, w/w) and seven seeds of each weed species were sown per pot. Two-week after emergence, pots were thinned to 5 equal-sized healthy plants per pot. Plants were watered daily and after 3 weeks they were flushed with halfstrength Hoagland nutrient solution (Hoagland and Arnon, 1950).When plants were 6-week-old, these were sprayed with 2, 4, and 6% (v/v) solution of Artemisia oil or distilled water (as parallel control) using a common garden sprayer at the rate of 100 ml/m2. For each seed type, a total of 20 pots comprising of 4 treatments and five replicates per treatment were maintained in a completely randomized design. One- and 7-days after spray (DAS), the treatedtest weed plants were examined for visible injury levels in termsof percent chlorotic and necrotic areas. Further, the leaves wereplucked for the determination of chlorophyll content and cellular respiratory activity.
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