All the samples were prepared in triplicates and analyzed three
times in HPLC. The peak areas were exported to a spreadsheet and
the quantities of the flavanones were calculated by applying regression equation and dilution factor. Finally, the data processing and
statistical analysis of the data was performed by SPSS version 16.0
(SPSS Inc., USA) software program. ANOVA was performed to analyze mean variations among treatments and results were expressed
in mean±SD.