2.4. In vitro fermentation
2.4.1. Pre-digestion of noodle samples
Prior to in vitro fermentation, all noodle samples were digested
by in vitro enzymatic digestion according to the method of Englyst
et al. (1992), with modifications. Briefly, 10 g of noodle powder
was added to 200 ml water in an Erlenmeyer flask. The suspension
was heated at 80 C for 5 min and then placed in a water bath at
37 C for 10 min to equilibrate. Sodium acetate buffer (0.1 M, pH
5.2, containing 4 mM CaCl2) was added and the mixture was
shaken well by hand. Alpha-amylase (9000 U/g starch; Sigma
A-3173) and amyloglucosidase (75 U/g starch; Sigma P7545) were