the stressed and non-stressed conditions were dependent on whether
the strains were non-acclimated (by never previously being exposed to
raw municipal wastewater) or acclimated (after 8 weeks exposure), respectively
to growth in municipal wastewater, as described previously
[3]. The mean spectra for each species and growth condition are shown
in Fig. S3. PCA scores plots of EMSC2 normalised cut-down FT-IR spectra
were generated for the nine algal species grown in non-stressed conditions
(Fig. 4a) and a combined plot of the species grown in nonstressed
and stressed conditions (Fig. 4b). As was expected from the
analysis of C. reinhardtii (Fig. 2) and D. tertiolecta (Fig. 3) separately, the
degree of individual species clustering was greater in non-stressed conditions
than in the combined plot of the species grown in all condition