Therefore the impact of reduced temperature on germination time was clearly demonstrated at 10 _C. The use of the asymmetric model for germination allowed very accurate estimations of the germination time, the standard deviation was ca. 0.20 h, Table 2. It is pointed out that, for all the temperatures, none of the spores had already germinated at 3/4 of the germination time. For example, at 10 _C, the percentage of germination was nil, Fig. 1, at 33.2 h, Table 2.